Want to find microsporidia that infect nematodes?

Step 1:

Locate the most rotting, disgusting fruit you can find.

IMG_4029.jpg

Step 2:

Convincing your lab to go apple picking, but conveniently forget to tell them that they will be picking apples off of the ground.

IMG_4045.jpg

Step 3:

Put the rotting fruit on plates and wait for worms to crawl out.

IMG_4413.jpg

Step 4:

Screen for microsporidia spores inside of the nematodes using the chitin-binding dye direct yellow 96.

hs1d_gta14_rep2_2.jpg

Thanks to everyone in the lab who helped gather samples last fall and rotation students Bryanna Meikle for help collecting and Mashiat Khan for screening through the samples!